Figure 3.

Fn-binding is localized to residues ¹⁵⁸Pro-His-Pro-Asp-Phe-Arg-Val¹⁶⁴ encoded within the P6 peptide. Binding of Fn to 96-well plates coated with peptides P1, P2, P5 and P6. Peptide-coated plates were incubated with serial dilutions of Fn and the amount of Fn bound was determined by ELISA. P1, P2 and P5 are 30mer peptides used above and P6 was designed to span predicted β strands and flanking disordered regions within the region shared by P1 and P2 (N150–V164). P5 was included as a negative control for Fn-binding. Data are displayed as mean values and standard deviations from triplicate samples. Statistical significance was determined at the 80 nM concentration by one-way ANOVA using Tukey's post-test (*P<0.05).