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Experimental phase memory curves for dual spin labeled E1 mutants (Tyr46-Val50/Ile66-Lys70) bearing two BSLs for 1% LMPG micelles (Tm = 1.9 ± 0.2 μs) (A) POPC/POPG=3:1 proteoliposomes (Tm = 0.9 ± 0.2 μs) (B), and lipodisq nanoparticles (Tm = 1.9 ± 0.2 μs) (C).
