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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: J Immunol. 2010 Sep 22;185(8):10.4049/jimmunol.1002151. doi: 10.4049/jimmunol.1002151

FIGURE 5.

FIGURE 5

Restoring positive selection rescues mTEC terminal differentiation. A, TetZap70 mice were fed dox for 14 d, resulting in the rescue of positive selection and the emergence of mature CD4+ and CD8+ thymocytes. B, Confocal analysis of untreated (left panels) or dox-treated (right panels) TetZap70 mice for CD4 (blue) and CD8 (green) together with either Aire (red, upper panels in B) or involucrin (red, lower panels). Original magnification ×40; scale bars, 20 μm. Arrow in B indicates a typical involucrin+ HC-like structure. C and D, Quantitative analysis of Aire+ and involucrin+ mTEC subsets, respectively, in untreated TetZap70 (red circles) and TetZap70 + dox (green circles). Results are representative of three separate experiments. **p < 0.01; ***p < 0.001.