FIGURE 4. Both CCR9− and CCR9+ TSP are detectable in the circulation of E11 nude mouse embryos.
A shows flow cytometric analysis of Annexin V staining in CD45+F4/80−PIRhigh CCR9− and CCR9+ cells from freshly isolated E12 WT C57BL/6 fetal liver. The number on the histogram indicates the percentage of cells positive for Annexin V staining. B shows flow cytometric analysis of CCR9 expression by CD45+F4/80− PIRhigh progenitors in the peripheral blood isolated from WT and nude E11 embryos.