Skip to main content
. 2013 Sep 30;11(10):3661–3675. doi: 10.3390/md11103661

Figure 4.

Figure 4

Methoxylated fatty acids inhibit LdTopIB by a CPT-independent mechanism. CPT-resistant LdTopIB CPTR [12,13] was assayed in the presence of DMSO (lane 0), 100 μM CPT, 100 μM acid 1, and 100 μM acid 2. Samples were incubated for 4 min at 25 °C, stopped with 1% SDS and digested for one extra hour at 37 °C in the presence of 1 mg/mL proteinase K. DNA was extracted with one volume of phenol-chloroform and samples were run on a 1% agarose gel containing ethidium bromide to a final concentration of 40 μg/mL in order to separate supercoiled and relaxed DNA. The nicked band corresponds to the stabilized cleavage complexes. The results are representative of three independent trials.