Figure 4.

Characteristics of colony-forming cells in the lung. (A) Using Methocult colony forming assay, numbers of colony derived from Lin⁺, Lin⁻/cKit⁻, or Lin⁻/cKit⁺ cells in the bone marrow (BM) and lung were counted (n = 4 per group). *P < 0.05 versus Lin⁻/cKit⁺. (B) Flow-cytometric analyses of Sca1 and cKit expression in BM Lin⁻ cells, lung Lin⁻ cells, or lung Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ cells are shown. The numbers of cKit⁺/Sca1⁻/Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ and cKit⁺/Sca1⁺/Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ cells in lung were enumerated and shown as percent of total lung cells (n = 4 per group). (C) The numbers of Sca1 and Ly6c positivity in lung Lin⁻/cKit⁺ cells were enumerated and shown as percent of total. (D) Numbers of colonies derived from sorted cKit⁺/Sca1⁺, cKit⁻/Sca1⁺, or cKit⁺/Sca1⁻ cells among the lung Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ cells are shown. *P < 0.05 versus cKit⁺/Sca1⁻. (E) GFP positivity rate in lung cKit⁺/Sca1⁻/Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ cells obtained from BM-chimera mice are shown (n = 4 per group). (F) Kinetics of cKit⁺/Sca1⁻/Lin⁻/Ly6c⁻/CD11c⁻/F4/80⁻ cells in the BM, lung, and spleen revealed significant increases occurred only in the lung in the BLM-treated mice (n = 3–5 per group). *P < 0.05 versus D0.