Fig. 5.

The expression of interferon (IFN)-γ and interleukin (IL)-17A mRNA and their master transcription factors [T-bet and retinoic acid-related orphan receptor (ROR)γt, respectively] in CD4⁺ T cells isolated from the mesenteric lymph nodes (MLNs) in mice with 1·5% dextran sodium sulphate (DSS)-induced colitis. CD4⁺ T cells were purified from MLNs with anti-CD4-coated magnetic beads. The mRNA levels were determined by quantitative real-time polymerase chain reaction (PCR). The columns represent the average ratio of each cytokine to 18S rRNA from 10 mice; *P < 0·05 versus control; #P < 0·05 versus trans fatty acid (TFA) group; †P < 0·05 versus DSS group. All results are expressed as the mean (± standard error of the mean) (n = 10 mice/group).