Figure 6.

Effect of external temperature and radial calibration on the measured sedimentation coefficients of the BSA monomer, all acquired at a nominal run temperature of 20 °C with the absorbance optical system. Shown are the measured time-corrected s_exp,τ-values prior to radial magnification correction (green asterisks), and the corresponding s_rad,τ-values after radial magnification correction (blue solid circles), respectively, plotted against the actual rotor temperature measured with the Thermochron iButton®. The lines represent the theoretical temperature-dependence, accounting for water viscosity, based on the average s_{20, rad, τ}–value of 4.333 S for all absorbance data shown including radial corrections (blue), and based on the average s_{20, τ}–value of 4.294 S for all absorbance data excluding radial corrections (green). The horizontal grey line indicates the average s_τ–value of 4.195 S for all absorbance data with neither temperature nor radial magnification corrections, as would be presumed based on the manufacturer's temperature and radial calibration at the used settings of 20 °C for all experiments.