Table 1.
Sedimentation coefficients measured on ten analytical ultracentrifuges, with and without corrections for temperature, radial magnification, time-stamp errors and scan velocity.
| Centrifuge | T (°C) | sexp (S) | visc factor (%) | s20 (S) | rad mag factor (%) | srad (S) | Time factora (%) | sτ (S) | s20,rad, τ (S) | s20,rad, τ,v (S) |
|---|---|---|---|---|---|---|---|---|---|---|
| Larry526b_IF | 17.15 | 4.405 | 7.11 | 4.718 | 0.48 | 4.426 | 9.93 | 4.007 | 4.312 | 4.312 |
| Larry526b_280 | 17.15 | 4.431 | 7.11 | 4.746 | 0.73 | 4.463 | 9.93 | 4.031 | 4.349 | 4.341 |
| Larry526_260 | 17.15 | 4.47 | 7.11 | 4.788 | 0.73 | 4.503 | 9.93 | 4.066 | 4.387 | 4.379 |
| Larry528_IF | 17.15 | 4.385 | 7.11 | 4.697 | 0.48 | 4.406 | 10.45 | 3.971 | 4.273 | 4.273 |
| Larry528_260 | 17.15 | 4.421 | 7.11 | 4.735 | 0.73 | 4.453 | 10.43 | 4.003 | 4.319 | 4.311 |
| Larry528_IF | 17.15 | 4.392 | 7.11 | 4.704 | 0.48 | 4.413 | 10.43 | 3.977 | 4.280 | 4.280 |
| Larry528_260 | 17.15 | 4.435 | 7.11 | 4.750 | 0.73 | 4.467 | 10.43 | 4.016 | 4.333 | 4.325 |
| Joe79_260 | 19.48 | 4.68 | 1.25 | 4.738 | 0.61 | 4.709 | 9.90 | 4.258 | 4.338 | 4.330 |
| Moe477 | 19.17 | 4.673 | 1.99 | 4.766 | 0.21 | 4.683 | 10.44 | 4.231 | 4.324 | 4.317 |
| Ted16_260 | 20.21 | 4.725 | −0.49 | 4.702 | 1.32 | 4.787 | 10.30 | 4.284 | 4.319 | 4.311 |
| Curly394_IF | 19.60 | 4.633 | 0.95 | 4.677 | 1.13 | 4.685 | 10.12 | 4.207 | 4.295 | 4.295 |
| Curly394_260 | 19.60 | 4.654 | 0.95 | 4.698 | 1.51 | 4.724 | 10.12 | 4.226 | 4.331 | 4.323 |
| Curly394_IF | 19.60 | 4.643 | 0.95 | 4.687 | 1.13 | 4.696 | 10.12 | 4.216 | 4.305 | 4.305 |
| Curly394_260 | 19.60 | 4.668 | 0.95 | 4.712 | 1.51 | 4.739 | 10.12 | 4.239 | 4.344 | 4.336 |
| Momo_IF | 19.66 | 4.660 | 0.81 | 4.698 | 1.00 | 4.707 | 10.28 | 4.226 | 4.302 | 4.302 |
| Momo_260 | 19.66 | 4.660 | 0.81 | 4.698 | 1.40 | 4.725 | 10.28 | 4.226 | 4.319 | 4.311 |
| Winnie_260 | 20.15 | 4.920 | −0.35 | 4.903 | −1.22 | 4.860 | 10.55 | 4.450 | 4.381 | 4.373 |
| Howie_IF | 19.54 | 4.675 | 1.10 | 4.726 | 1.45 | 4.743 | 10.30 | 4.317 | 4.347 | 4.347 |
| Shemp319_280 | 21.31 | 4.663 | −3.2 | 4.514 | 6.13 | 4.949 | 10.35 | 4.226 | 4.342 | 4.334 |
| Shemp319_IF | 21.31 | 4.870 | −3.2 | 4.714 | 0.29 | 4.884 | 10.35 | 4.413 | 4.284 | 4.284 |
| Shemp320_280 | 21.31 | 4.553 | −3.2 | 4.407 | 7.41 | 4.890 | 10.46 | 4.122 | 4.286 | 4.278 |
| Shemp320_IF | 21.31 | 4.811 | −3.2 | 4.657 | 0.76 | 4.847 | 10.48 | 4.355 | 4.248 | 4.248 |
| Clotho_IF Clotho_280 | 19.66 19.66 | 4.243 4.317 | 0.81 0.81 | 4.277 4.352 | 0.15 -0.44 | 4.249 4.298 | 0.30 0.29 | 4.230 4.305 | 4.271 4.320 | 4.271 4.312 |
|
| ||||||||||
| Average | 4.583 | 1.98 | 4.669 | 1.20 | 4.638 | 4.192 | 4.317 | 4.313 | ||
| standard deviation(%) | 3.83 | 4.25 | 3.04 | 0.69 | 4.31 | 3.29 | 0.78 | 0.73 | ||
| maximum range | −2.85 – | 4.243 – | −3.2 – | −1.22 – | 0.29 – | 3.977 – | 4.248 – | 4.271 – | ||
|
| ||||||||||
| 1.31 | 4.920 | 7.11 | 7.41 | 10.45 | 4.450 | 4.387 | 4.379 | |||
a
Except for Clotho, all instruments utilized Version 6 (Firmware 5.06) of the Proteome Lab XLA/I data acquisition software. In the case of Clotho, Version 5.8 (Firmware 4.21) was used.