Skip to main content
. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Cell. 2010 Oct 1;143(1):10.1016/j.cell.2010.08.039. doi: 10.1016/j.cell.2010.08.039

Figure 2. Fibroblasts Mediate Schwann Cell Sorting by Modifying Schwann Cell Behavior.

Figure 2

(A) Immunofluorescence images of S100β-labeled SC cultured in the absence (SC) or presence (SC+Fb) of Fb, 6 and 24 hr after seeding.

(B) Immunofluorescence staining for S100β (S100) and fibronectin (Fibro) of SC monocultures (SC) or SC/Fb cocutures (SC+Fb). Scale bars represent 50 μm.

(C) Still images from time-lapse microscopy experiments of SC cultured alone (SC) or SC cocultured with Fb (SC+Fb). Shown is one example of SC repelling each other in the absence of Fb (top panels) and one example of SC adhering to one another in the presence of Fb (bottom panels). Numbers in white indicate elapsed time in minutes after plating.

(D) Quantification of SC behavior in movies described in (C). The bar graph represents the average number (±SD) of repulsive and adhesive events per condition. Three independent experiments were quantified by counting a minimum of 20 cells per video.

See also Figure S2 and Movies S1, S2, and S3.

HHS Vulnerability Disclosure