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Viral spreading assay. Confluent BHK-21 cells were infected with rLa-cell, rLa-cell/TPCK, rLa-EBOVGP-cell, rLa-ZEBOVGP-cell/TPCK, rLa-egg, or rLa-EBOVGP-egg at MOIs of 0.05. The cells were fixed at different time points and detected with chicken anti-NDV antiserum, then incubated with an FITC-conjugated rabbit anti-chicken antibody.
