Figure 4.

Chemical inhibition of virus internalization pathways. BHK-21 cells were pretreated with (right panel) or without (left panel) NA alone or together with inhibitor 10 μM IPA3 or 10 mM MβCD or a mixture of the two chemicals at 37°C for 1 h. The cells were washed and infected with 5 × 10² TCID₅₀ of rLa-egg, rLa-EBOVGP-egg, rLa-cell/TPCK rLa-EBOVGP-cell, or rLa-EBOVGP-cell/TPCK at 4°C for 1 h in the presence of the chemical(s). The cells were then washed and incubated at 37°C. At 7 h after infection, the cells were washed, fixed, permeabilized, and immunostained with chicken anti-NDV antiserum, then incubated with a FITC-conjugated rabbit anti-chicken antibody.