Figure 2. The K-mer distribution and contig frequency analysis.

(a) Seventeen k-mer depth distribution of whole-genome Illumina reads. Two peaks at 16 and 30 were identified. (b) Two peaks of Cvg 7 and 17 were identified in the distribution of contig length and depth. These results indicated that the two haploid nuclei of Pst urediniospore are highly heterozygous. The haploid genome size can be estimated using a modified Lander–Waterman algorithm based on K-mer analysis (Supplementary Notes 1 and 2). K-mer, a unique sequence of k nucleotides long; Cvg, coverage.