Figure 7. Assays for three putative Pst SP genes specifically upregulated during infection of Berberis leaves.

Pst genes striiformis_9106, 9305, and 1374 are putative SP genes that are specifically upregulated at the pycnial stage in the infected Berberis leaves. RNA samples were isolated from urediniospores (US), germ tubes (GT), infected wheat leaves at 24 h post inoculation (h.p.i.) or 120 h.p.i. and infected berberis (Berberis shensiana Ahrendt) leaves (BE) at 11 days post inoculation (11 d.p.i.), respectively. The relative expression levels were calculated by the comparative Ct method with the elongation factor-1 gene of Pst as the internal standard. Means and s.e. were calculated from three replicates. Error bars represent the s.d. of the mean.