Table 1. Primers used in this study.
| Primer | 5′→3′ Sequence |
|---|---|
| 3′UTR PbAMA1 fw | GGGCTCGAGAAAAATAGCCATTGATTAA |
| 3′UTR PbAMA1 rv | GGGCTGCAGTACCGATTAAGAGAAATGCT |
| 5′UTR PbAMA1 fw | GGGGAATTCCTATAAATACGCTATATGCA |
| 5′UTR PbAMA1 rv | GGGGAGCTCTTTTATATCGTTTTATTTTA |
| 5′Pbama1-probe fw | CTTATTTCACAGCTAGCCAT |
| 5′Pbama1-probe rv | CAATTACCATACAATACTTATAT |
| Pa (PbAMA1 cds fw) | GAATAATGATGTTGAAATAAAAG |
| Pb (PbAMA1 3′UTR rv) | TTTAATTTAATGCCATCTATAATG |
| Pc (hDHFR fw) | GGTGAGCATTTAAAGCACAA |
| P3 (5′UTR TgAMA1 fw) | GAGGGCCCATGCATGGATCGTATCGTACGAGACTAACG |
| 5′UTR TgAMA1 rv | GAGGGCCCGAGACCCAATCAGATGTTAGCAAGC |
| 3′UTR TgAMA1 fw | GAGAGCTCAGTCAGAAGAAACTGGGTACAGTTTTCCC |
| 3′UTR TgAMA1 rv | GAGAGCTCCCCGGGGGCTTGACTAAACATTCCTCTCCCTATCAC |
| TgAMA1 ORF fw | GAGAATTCTAAAATGATCTGTTCAATCATGGGAGGCTTGC |
| P2′ (TgAMA1 ORF rv) | GATTAATTAACTAGTAATCCCCCTCGACCATAACATGTG |
| P1 (5′UTR TgAMA1out fw) | GGACTCAAGCTTTACGGGATTTGCC |
| P1′ (P5RT70 rv) | CCGAATCTATAACTTCGTATAATGTATGCTATACGAAGTTATAGAAAAAATGCCAACGAGTAGTTTTCCGCAAGAACTT |
| P2 (TgAMA1int fw) | GCGGCAATTGCTGGACTCGCCGTAGGAG |
| P3′ (YFP rv) | TGCATTTACTTGTACAGCTCG |
Fw, forward; ORF, open reading frame; rv, reverse; UTR, untranslated region.