Table 1. Primers used in this study.
Primer | 5′→3′ Sequence |
---|---|
3′UTR PbAMA1 fw | GGGCTCGAGAAAAATAGCCATTGATTAA |
3′UTR PbAMA1 rv | GGGCTGCAGTACCGATTAAGAGAAATGCT |
5′UTR PbAMA1 fw | GGGGAATTCCTATAAATACGCTATATGCA |
5′UTR PbAMA1 rv | GGGGAGCTCTTTTATATCGTTTTATTTTA |
5′Pbama1-probe fw | CTTATTTCACAGCTAGCCAT |
5′Pbama1-probe rv | CAATTACCATACAATACTTATAT |
Pa (PbAMA1 cds fw) | GAATAATGATGTTGAAATAAAAG |
Pb (PbAMA1 3′UTR rv) | TTTAATTTAATGCCATCTATAATG |
Pc (hDHFR fw) | GGTGAGCATTTAAAGCACAA |
P3 (5′UTR TgAMA1 fw) | GAGGGCCCATGCATGGATCGTATCGTACGAGACTAACG |
5′UTR TgAMA1 rv | GAGGGCCCGAGACCCAATCAGATGTTAGCAAGC |
3′UTR TgAMA1 fw | GAGAGCTCAGTCAGAAGAAACTGGGTACAGTTTTCCC |
3′UTR TgAMA1 rv | GAGAGCTCCCCGGGGGCTTGACTAAACATTCCTCTCCCTATCAC |
TgAMA1 ORF fw | GAGAATTCTAAAATGATCTGTTCAATCATGGGAGGCTTGC |
P2′ (TgAMA1 ORF rv) | GATTAATTAACTAGTAATCCCCCTCGACCATAACATGTG |
P1 (5′UTR TgAMA1out fw) | GGACTCAAGCTTTACGGGATTTGCC |
P1′ (P5RT70 rv) | CCGAATCTATAACTTCGTATAATGTATGCTATACGAAGTTATAGAAAAAATGCCAACGAGTAGTTTTCCGCAAGAACTT |
P2 (TgAMA1int fw) | GCGGCAATTGCTGGACTCGCCGTAGGAG |
P3′ (YFP rv) | TGCATTTACTTGTACAGCTCG |
Fw, forward; ORF, open reading frame; rv, reverse; UTR, untranslated region.