Fig. 6.

Mitochondrial respiration and hydrogen peroxide production in muscle from CHOW-, MCFA-, and LCFA-fed mice. Mitochondrial oxygen consumption was measured in state 2, state 3, and state 4 using succinate (A) or palmitoyl carnitine (B) as a substrate. Hydrogen peroxide production was measured in the presence of succinate, succinate plus rotenone (Rot), and palmitoyl carnitine (C). H₂O₂ production and state 2 oxygen consumption were used to calculate the percentage of electrons which leak out of sequence producing superoxide and subsequently hydrogen peroxide, the free radical leak (D). Shown are means ± SEM; n = 6–8 per diet group; *P < 0.05, **P < 0.01, and ***P < 0.001.