Fig. 5.

Transient expression of CCT-Δ28 in CHO MT58 cells increases PtdCho synthesis. A: Total lysates of CHO-MT58 cells transiently expressing CCTα or CCTα-Δ28 were immunoblotted with a V5 monoclonal antibody. B: Transiently transfected CHO-MT58 cells were immunostained using V5 monoclonal antibody and Alexa Fluor-488 antibodies. Hoechst 33258 was used to counterstain the nucleus. C, D: CHO-MT58 cells transiently expressing V5-tagged CCTα or CCTα-Δ28-V5 were cultured at 42°C. After 48 h, cells were labeled with [³H]choline (2 μCi/ml) for 4 h and PtdCho (C) and choline metabolites (D) were quantified. Choline incorporation was normalized to expression of CCTα and CCTα-Δ28 by densitometry of immunoblots of total cell lysates. Results are the mean and standard deviation for three experiments. *P < 0.05 using unpaired t-test compared with CCTα expressing cells.