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. 2013 Dec;54(12):3373–3384. doi: 10.1194/jlr.M041434

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Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 6. — Kinetic analysis of CCTα-Δ28. Soluble (A) and membrane fractions (B) from CHO-MT58 cells (cultured at 42°C) transiently expressing CCTα or CCTα-Δ28 were assayed for CCTα activity at increasing concentrations of CTP (mM). Soluble CCTα activity was assayed in the presence of PtdCho/oleate vesicles. The activity of membrane fractions was assayed in the absence of PtdCho/oleate vesicles. Enzyme activity was normalized to expression of CCTα or CCTα-Δ28 in each fraction by immunoblotting and densitometry. K_m and V_max values (see inserts) are the mean and standard deviation for three experiments.