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. 2013 Dec;54(12):3385–3393. doi: 10.1194/jlr.M041749

TABLE 1.

Percent SM in exchange vesicles

Sample Composition SM Percent by TLC
SMo/POPG i 57.9 ± 4.3 (4)
SMo/DOPG i 57.5 ± 2.1 (2)
SMo/soy PI i 59.9 ± 1.7 (5)
SMo/TOCL i 67.8 ± 4.1 (2)
SMo/POPC:DOPA 2:1 i 62.2 ± 2.1 (2)
SMo/POPS i 59.1 ± 4.5 (2)
SMo/POPE:POPG 2:1 i 63.2 ± 3.8 (2)
SMo/POPE:DOPG 2:1 i 59.1 ± 2.4 (2)
SMo/POPE:soy PI 2:1 i 60.5 ± 2.6 (2)
SMo/POPE:TOCL 2:1 i 58.6 ± 3.5 (2)
SMo/POPE:POPS 2:1 i 57.6 ± 2.6 (2)

Percent of SM in exchange vesicles was calculated from HP-TLC analysis of Sepharose CL2B fractions (details in Materials and Methods). Average (mean) and range derived from duplicate experiments or SD when n > 2 is shown. The number of experiments is shown in parentheses. o, outer leaflet lipid; i, inner leaflet lipid.