Fig. 8.

Addition of unsaturated PA or unsaturated LPA to mouse chow produces changes in gene expression in the small intestine similar to those seen after feeding WD in LDLR^−/− mice. Female LDLR^−/− mice, age 7–8 months (n = 4–6 per group), were fed mouse chow (Chow), WD, or chow supplemented with 1 μg of PA or LPA (18:0, 18:2, or 20:4) per gram chow. After 3 weeks the small intestine was harvested from each mouse, RNA was isolated from the jejunum, and RT-qPCR for the genes shown in Fig. 4 was performed. The data shown are mean ± SD. *P < 0.05; **P < 0.01; ***P ≤ 0.001.