Fig. 10.

Effect of native FADS3 extinction and recombinant FADS3 expression on the conversion of trans-vaccenate into the suspected trans11,cis13-CLA in rat hepatocytes. Hepatocytes were transfected with siRNA control or siRNA against Fads3 for 24 h and incubated for a further 24 h with TVA. Similarly, hepatocytes were transfected with the pCMV empty or pCMV/FADS3 for 24 h before incubation with TVA. (A) GC-MS profiles of FAMEs extracted from cultured rat hepatocytes in each experimental condition. For this set of experiments, the GC column, which was previously used for all the other GC-MS analyses, was changed, and isomers of eicosenoic acid (20:1) were eluted in the same region of the chromatogram as the suspected trans11,cis13-CLA. (B, C) Quantification (percentage of total FA) of the most interesting FAs (TVA, cis9,trans11-CLA, cis6,trans11-18:2, and the suspected trans11,cis13-CLA) after GC-MS analysis of cellular FAMEs extracted from rat hepatocytes in each experimental condition. Data are presented as mean ± SD (n = 4).