Fig. 2.
GC-MS profiles of FAMEs extracted from COS-7 cells expressing rat FADS1, FADS2, or FADS3 and incubated with TVA. Control COS-7 cells and COS-7 cells transiently expressing the recombinant FADS proteins were cultivated for 24 h with albumin-bound TVA (200 µM). The identity of each important FA is indicated above its respective peak. Rumenic acid (Rt = 27.5 min) was detected in all the experimental conditions (control cells and transfected cells). Three different and unknown octadecadienoic acids (with FAME molecular ions at m/z 294) were produced in COS-7 cells expressing FADS1 (Rt = 21.9 min), FADS2 (Rt = 22.1 min,) and FADS3 (Rt = 28.2 min). Representative example of n = 5 distinct experiments.