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. 2013 Dec;54(12):3438–3452. doi: 10.1194/jlr.M042572

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Copyright © 2013 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Identification of the unknown octadecadienoic acid produced from trans-vaccenate in cells expressing rat FADS3 as a Δ11,13-conjugated linoleic acid isomer. (A) Electron-impact mass spectrum of the DMOX derivative of the unknown ¹³C-octadecadienoic acid obtained after incubation of COS-7 cells expressing FADS3 with [1-¹³C]TVA. (B) GC-MS profile of the FAMEs extracted from COS-7 cells expressing FADS3 and incubated with TVA compared with commercial rumenic acid and a commercial mixture of cis11,trans13-18:2, cis11,cis13-18:2, and trans11,trans13-18:2 as FAME standards. (C) Electron-impact mass spectrum of the DMOX derivative of the commercial cis11,trans13-18:2.