Fig. 6.
GC-MS profile of cellular FAMEs extracted from COS-7 cells expressing rat FADS1, FADS2, or FADS3 and incubated with cis-vaccenate. Control COS-7 cells and COS-7 cells transiently expressing the recombinant FADS proteins were cultivated for 24 h with albumin-bound cis-vaccenate (200 µM). The identity of each important FA is indicated above its respective peak. It shows that only the cells expressing FADS1, but not FADS2 or FADS3, were able to desaturate CVA into an octadecadienoic acid (Rt = 22.4 min), which was identified as the cis5,cis11-18:2 by the NIST database. Representative example of n = 3 distinct experiments.