Fig. 5.

Effects of DHA on viability of BC cells. The effect on cell viability of DHA in BT-474, SK-BR-3, and E0771 cells is assessed and quantified by MTT assay. Cells were treated with various concentrations of DHA. BT-474, SK-BR-3, and E0771 cells were seeded and cultured for 24 h in 96-well plates at a density of 3 × 10³ cells per well and cultured in medium supplemented with 10% FBS. After this period, the cells were washed twice with PBS and the medium was replaced with fresh medium with 0.5% FBS containing DHA at increasing concentrations (20–100 μM) for a further 72 h. The number of viable cells exposed to DHA was evaluated by a colorimetric MTT assay. Data represent the mean of eight values and results and are expressed as viability in comparison with controls (100%).