Fig. 6.

DHA inhibits HER2/HER3 expression and subsequent signaling pathway in SK-BR-3, BT-474, and E0771 cell lines. SK-BR-3 (A) and BT-474 (B) cells were treated with DHA at 40 and 80 μM in medium supplemented with 0.5% FBS for 24, 48, and 72 h. E0771 (C) cells were treated with DHA at 40 and 80 μM in medium supplemented with 0.5% FBS for 24 and 48 h. Heregulin β-1 (50 ng/ml) was added 1 h before harvest and lysates were immunoblotted as indicated for HER2, p-HER2, HER3, p-HER3, E-cadherin, β-catenin, p-Akt, p-GSK-3β, c-Myc, and β-actin protein expression.