Figure 7. Effect of Substance P reverse (SPrev) and Substance P (SP) (10⁻⁶ M) on the biofilm formation activity of Bacillus cereus.

The biofilm formation was observed after 2, 5 and 24 h. (A) Two dimensions (2D) and reconstructed three-dimensions (3D) and ortho cuts (3D/z) images showed that the density of the biofilm was essentially unchanged. (B) In contrast, the thickness of the biofilm was significantly increased after 5 and 24 h inclubation with SP (NS = non significant;  = p<0.01;  = p<0.001).