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. 2013 Nov 8;8(11):e78639. doi: 10.1371/journal.pone.0078639

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© 2013 Junqueira-Kipnis et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Lung and spleen single-cell suspensions from vaccinated and control mice were restimulated with CMX or medium, and the CD4⁺ and CD8⁺ IFN-γ-positive T cells were analyzed by flow cytometry. Lymphocytes were selected by their size and granularity and the CD4⁺ cells were gated and analyzed for IFN expression. A and B show CD4⁺ IFN-γ-positive cells from the spleen and lungs, respectively. C and D show CD8⁺ IFN-γ-positive cells (n = 4; *p<0.05) from the spleen and lungs, respectively. The results are representative of two independent experiments.