Figure. 4. Prx augmentation of ADCVI dependent and independent HIV inhibition.

Purified human CD4⁺ T cells were infected with SIVmac251 at a multiplicity of infection of 0.01. Human NK cells were simultaneously purified from the same donor and cultured separately. Two days after infection, CD4⁺ T cells and purified NK cells were washed extensively and resuspended in 96-well plates at 5 × 10⁴ CD4⁺ T cells and 1 × 10⁵ NK cells per well. Pooled heat-inactivated plasma from 4 rhesus monkeys chronically-infected with SIVmac251 was added at a final dilution of 1:100 or 1:250. Prx was added at the indicated concentrations. Four days later, viral titers in culture supernatants were quantified by p27 ELISA. Inhibition was calculated from controls wells lacking Prx. ADCVI independent inhibition was measured without the addition of NK cells (Prx alone). Experiments were repeated multiple times in separate donors with representative results from a 1:250 dilution of plasma demonstrated. Error bars represent standard error of the mean (S. E.). Some are too small to show.