Figure 3.

Validation of ten differentially expressed genes using quantitative PCR on independently generated biological replicate sample sets as templates. Transcripts were randomly selected for validation and their expression considered significantly regulated (*) if p < .05 using a 1-way ANOVA with Bonferroni post-test analysis (Graphpad Prism v.5.0a). Expression values as cycle thresholds were normalized to those of a house keeping gene, BBOV_III004820, thus, the final data are represented as cycle threshold ratio (CT ratio) where the lower the ratio value, the higher the expression. III 010740, gene encoding for 1-deoxy-D-xylose-5-phosphate reductoisomerase; III006460 and III006500, genes encoding for spherical body protein 2 truncated copies 7 and 9 respectively; II007790, gene encoding for a hypothetical protein; III006070, III001500, I004520 and III003100, genes encoding for variant erythrocyte surface (ves) α subunits; III006080 and IV001490, genes encoding for ves β subunits and III002360, gene encoding for a putative membrane protein.