Figure 7.

Validation of RNA-sequencing data with quantitative PCR using independently generated biological replicate sample set. Only highly regulated transcripts were randomly selected for validation and considered significantly (*) expressed if p < .05 using a 1-way ANOVA with Bonferroni post-test analysis (Graphpad Prism v.5.0a). Expression values as cycle thresholds were normalized to those of a house keeping gene, BBOV_III004820, thus, the final data are represented as cycle threshold ratio (CT ratio) where the lower the ratio value, the higher the expression. I003010, gene encoding for merozoite surface antigen 2a1; III000400, gene encoding for a small open reading frame protein (SmORF); III002360, gene encoding for a membrane protein; III004490, gene encoding for a hypothetical protein; III005840, III006460 and III006540 were genes encoding for spherical body protein 2 truncated copies 5, 7 and 11, respectively; IV012120, gene encoding for a membrane protein.