Figure 4. Control of PR cell fates by spineless.
a, In whole-mount retinas from PanR7>ss flies, all R7 cells express Rh4 (cyan), whereas Rh3 (red) is absent (left panel). Late expression of ss in R7 has no effect on the ratio between Rh5 (blue) and Rh6 (green) expression (right panel). b, Section of a PanR7>ss eye. As the fate of R8 is not affected by late ss expression in R7 cells, there is a great number of ‘odd-coupled’ Rh4 (cyan)/Rh5 (blue) ommatidia. The arrow indicates the DRA. c, In whole-mount retinas from PanR7>ss flies on a ss mutant background, Rh3 (red) is not expressed, while every R7 cell expresses Rh4 (cyan) (left panel). Late overexpression of ss in all R7 cells on a ss mutant background does not lead to a reprogramming of R8 cells as most R8 cells express Rh5 (blue) and few dorsal R8 express Rh6 (green) (right panel).