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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: J Clin Cell Immunol. 2011 Nov 15;Suppl 1:003. doi: 10.4172/2155-9899.S1-003

Figure 2. Calpain-1 is activated by calcium during eosinophil apoptosis.

Figure 2

(A) Calpain-1 immunoblot analysis in PBEos lysed immediately (0), left untreated (R), or treated as shown. The p80 and p76 calpain signals were quantified with Image J software 1.34S (NIH) and ratio calculated and normalized to that observed for control untreated PBEos. (B) Immunoblot analysis of α-spectrin in eosinophils treated as (A). (C) PBEos were treated as shown, lysed and calpain-1 autolysis was determined by immunoblot. ** p<0.01; # p< 0.05 (one-way ANOVA). Data representative of at least 3 independent experiments from different donors and expressed as the mean ± SEM.