FIGURE 2:

SENP1 and SENP2 association with NPCs is determined by N-terminal targeting signals. HeLa cells were transfected with constructs coding for GFP-tagged SENP1, SENP2, or the indicated chimeras, fixed with formaldehyde, and permeabilized with TNX-100. Cells were stained with monoclonal antibody (mAb) 414 to detect nuclear pore complexes and imaged by immunofluorescence microscopy. DNA was stained with DAPI. Bar, 10 μm.