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. 2013 Nov 15;24(22):3483–3495. doi: 10.1091/mbc.E13-05-0230

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© 2013 Cubeñas-Potts et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 7: — Immunofluorescence microscopy analysis of endogenous SENP1 and SENP2. HeLa cells were fixed, permeabilized, stained, and analyzed by immunofluorescence microscopy. DNA was labeled with DAPI. Cells were colabeled with (A) SENP1- or SENP2-specific antibodies and mAb 414 to detect nuclear pore complexes in interphase, (B) SENP1- or SENP2-specific antibodies and Hec1-specific antibodies in mitosis, and (C) SENP1- and tubulin-specific antibodies in mitosis. Bar, 10 μm.