FIGURE 10:

Analysis of protein methylation in smyd1b-knockdown embryos by Western blot. Lanes 1 and 2, Coomassie blue staining showing protein expression in wild-type (WT) control and smyd1b-knockdown embryos at 72 hpf. Lanes 3 and 4, protein methylation in WT control and smyd1b-knockdown embryos at 72 hpf by Western blot using anti–methyl lysine antibody. Lanes 5 and 6, myosin heavy chain protein levels in WT control and smyd1b-knockdown embryos at 72 hpf by Western blot using MF-20 antibody. γ-Tubulin was probed as a loading control.