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. 2013 Nov 15;24(22):3545–3556. doi: 10.1091/mbc.E13-06-0332

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© 2013 Bernardi et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 8: — Disrupting YOD1 activity affects TCRα degradation without altering its ubiquitination level. (A) Cells transfected with TCRα-HA and cotransfected with YFP, FLAG-WT YOD1, or FLAG-C160S YOD1 were incubated with cycloheximide for the indicated time. The resulting WCLs were immunoblotted with an HA antibody. (B) As in Figure 5B. (C) As in (A), except cells were transfected with scrambled siRNA or YOD1 #1 siRNA. (D) The TCRα-HA band intensity in (C) was quantified as in Figure 2C. Mean of at least three independent experiments. Error bars: ±SD. (E) As in (B), except cells were transfected with scrambled siRNA or YOD1 #1 siRNA. (F) As in (C), except cells were transfected with NHK-HA.