Fig. 1.

Intestinal proglucagon mRNA expression in young and adult ad libitum versus dietary-restricted rats. Results represent the mean±SEM for 8 adult AL (ad libitum), 9 adult DR (dietary restriction), and 6 young AL rats. Gene expression is expressed as a fold change from the young AL rats for which the value is set at 1.0. Using the 2−ΔΔCT calculation each gene is corrected against the housekeeping gene actin. Values with different letters represent a difference between groups within a given intestinal segment (p<0.05). Where no letters are present there are no differences between groups.