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. Author manuscript; available in PMC: 2013 Nov 13.
Published in final edited form as: Nutrition. 2008 Nov 26;25(3):340–349. doi: 10.1016/j.nut.2008.08.012

Fig. 2.

Fig. 2

Effects of leucine, isoleucine, and valine on in vitro mRNA expression of (A) i-FABP, (B) FATP4, and (C) NPC1L1. NCI-H716 cells (2 × 106) were incubated for 42 h with Dulbecco’s Minimal Eagle’s Medium plus 0.2% bovine serum albumin with or without one of the treatments (w/v). Polymerase chain reaction products for genes of interest were normalized to β-actin mRNA as a control. Due to lower solubility, leucine concentrations are 1.5% and 2.0% instead of 2.0% and 3.0%. Values are means ± SEMs of four experiments. Treatments with different letters are significantly different (P < 0.05). FATP4, fatty acid transport protein-4; i-FAB, intestinal-type fatty acid binding protein; NPC1L1, Niemann-Pick C-1–like-1 protein.