Table 2.
Groups of micro-organisms, primers and bacteria genomic DNA standards for quantitative PCR
| Groups | Primers (F and R) | Genomic DNA | References |
|---|---|---|---|
| Total bacteria | F: ACTCCTACGGGAGGCAG R: GTATTACCGCGGCTGCTG |
Escherichia coli | Amann et al.(60), Liu et al.(61) |
| Firmicutes | |||
| Clostridium leptum (group IV)* | F: GCACAAGCAGTGGAGT R: CTTCCTCCGTTTGTCAA |
Clostridium leptum | Matsuki et al.(62) |
| Clostridium coccoides (group XIV) | F: ACTCCTACGGGAGGCAGC R: GCTTCTTAGTCARGTACCG |
Ruminococcus productus | Amann et al.(60), Franks et al.(63) |
| Lactobacillus spp. | F: GAGGCAGCAGTAGGGAATCTTC R: GGCCAGTTACTACCTCTATCCTTCTTC |
Lactobacillus jensenii | Delroisee et al.(64) |
| Bacteriodetes | |||
| Bacteroides/Prevotella | F: TCCTACGGGAGGCAGCAGT R: CAATCGGAGTTCTTCGTG |
Bacteroides thetaiotaomicron | Bernhard & Field(65), Nadkarni et al.(66) |
| Actinobacteria | |||
| Bifidobacterium spp. | F: CGCGTCYGGTGTGAAAG R: CCCCACATCCAGCATCCA |
Bifidobacterium adolescentis | Delroisee et al.(64) |
| Proteobacteria | |||
| Enterobacteriaceae | F: CATTGACGTTACCCGCAGAAGAAGC R: CTCTACGAGACTCAAGCTTGC |
Escherichia coli | Bartosch et al.(67) |
F, forward; R, reverse.
*
The primer set for C. leptum covers close relatives of C. leptum and does not cover the genus Rumminicocus.