Figure 1. UTX is required for cell survival following IR exposure in Kc cells.

(A) Cell counts at 2 and 4 days following RNAi treatment are indicated. Note that at 2 days, there is no difference in the growth rate of utx RNAi-treated compared to control (Ctrl) RNAi-treated cells. The asterisk indicates P<0.05 compared with the Ctrl RNAi group on the same day, as determined by two-way ANOVA followed by a post-hoc Tukey’s test for comparisons between groups. (B) utx RNAi-treated cells display significantly reduced cell counts compared to control RNAi-treated cells and over-expression wild type UTX could rescue RNAi effect but not mutant UTX after IR exposure. 4 days RNAi-treated cells were irradiated at doses of 4 and 8 Gy and examined relative cell viability after 2 days.(C) Western blot analysis of UTX expression confirming the efficiency of utx RNAi-mediated knockdown and over-expression of UTX. (D) IR treatment causes no detectable change in UTX protein levels. β-Tubulin (Tub) levels were used as loading controls. All of the data are representative of at least three independent experiments with similar results. Error bars indicate standard deviations from triplicate sets of the presented experiment.