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. 2013 Nov 12;8(11):e78725. doi: 10.1371/journal.pone.0078725

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© 2013 Mukhopadhyay et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Agar colonies were obtained by culture of sorted cells from mouse mammary carcinoma Cl66 cells cultured in soft-agar containing complete DMEM media. Stem-type cells formed higher numbers of agar colonies than non-stem type cells. ALDH1⁺ and ALDH1⁻ formed more agar colonies than SP cells and SP cells formed more agar colonies than non-SP cells. Soft agar colony formation was much lower in CD34^high and CD49f^high cell populations. Matrigel™ increased the size of agar colonies of all cell populations, indicating that all cell populations responded to the growth factors present in Matrigel™. With Matrigel™ indicated by +M. All data presented are the average of 2 independent experiments.