Figure 6. Steady state Trp fluorescence anisotropy measurements.

OmpX^HN (A) and OmpX^M (B) refolded using heat shock in 10 mM LDAO were subjected to GdmHCl denaturation for 24 h and anisotropy values were recorded. Fits to a two-state equation for OmpX^HN and a three-state equation for OmpX^M, are shown as solid lines. Note that the unfolding of OmpX^M follows a three state transition, while OmpX^HN fits well to a two-state, as indicated by the residuals (shown below each curve as standard deviation (Std. Dev.)).