Figure 4. α1(V) peptide binding to HLA-DQ2, HLA-DQ6 and mouse H-2 I-A^b.

ProImmune REVEAL MHC-peptide binding assay screen of 5-amino acid overlapping peptide library (101 total peptides) of the α1(V) helical domain to HLA-DQ2 (A), HLA-DQ6 (B) and H-2 I-A^b (C). Each MHC:peptide binding score is based on known binding of a positive control peptide and a weaker binding positive control peptide. MHC:peptide binding scores above 12% are considered positive and have potential for immunological activity. Lack of bar for a peptide represents a score of 0%. Peptide number corresponds to the location in the α1(V) AA sequence. Green shaded areas indicate where HLA-DQ2, -DQ6 and H-2 I-A^b similarly bound within the helical domain.