Figure 6. Deletion in the agnogene prevents VP1 expression.

Western blots for VP1 were done as described in Figure 2. (A) VP1 levels in Cos-7 cell lysate 14 days post-transfection. (B) VP1 levels in Cos-7 cells 21 days post-transfection. Levels of VP1 expression are drastically reduced in Mad-1 C-Agno, and expression is rescued by the full length agnogene in CPN M-Agno. Deletion of the agnogene Mad-1 Del results in a greater decrease in VP1 expression than just the prevention of the protein expression in Mad-1 Pt. Blots are representative of 3 or 4 independent experiments.