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. 2013 Nov 13;8(11):e78618. doi: 10.1371/journal.pone.0078618

Figure 1. Preparation and treatment time lines for human and murine DCs.

Figure 1

(A) Human iDCs were obtained from CD14+ cells cultured in GM-CSF and IL-4 for 8 days. Human mDCs were obtained from CD14+ cells stimulated with GM-CSF and IL-4 for 8 days and stimulated by TNF-α in the last 2 days. Human GDF-15 treated DCs were obtained from CD14+ cells cultured in GM-CSF, IL-4 and GDF-15 for 8 days and stimulated by TNF-α in the last 2 days. (B) Murine iDCs were obtained from bone marrow cells cultured in GM-CSF and IL-4 for 8 days. Murine mDCs were obtained from bone marrow cells cultured in GM-CSF and IL-4 for 8 days and stimulated by CT26 cells lysate in the last 2 days. Murine GDF-15 treated DCs were obtained from bone marrow cells cultured in GM-CSF, IL-4 and GDF-15 for 8 days and stimulated by CT26 cells lysate in the last 2 days.