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. 2013 Nov 13;8(11):e78618. doi: 10.1371/journal.pone.0078618

Figure 2. Confirmation of in vitro binding by cell-based ELISA.

Figure 2

(A) Identification of the binding selectivity of the 38 clones by cell-based ELISA. The iDCs (CD14+ cells and mDCs were used as negative controls) were incubated with the amplified T7 phage in 96-well plates at 37°C for 1 h. Phage clones binding to cells were detected by the T7 Tail Fiber monoclonal antibody and following sheep anti-mouse IgG conjugated with HRP. (B) Specificity of the GDF-15-expressing phage to iDCs. Control phage: uncombined phage in the first round of panning. All assays were carried out in triplicate and the error bars indicate standard deviation.