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. 2013 Nov 13;8(11):e78618. doi: 10.1371/journal.pone.0078618

Figure 4. GDF-15 affects the phenotypes of DCs.

Figure 4

(A) Quantitative PCR analysis of DC phenotypes based on the expression of CD83, CD86 and HLA-DR. The quantitative values for the genes of interest were normalized using the housekeeping gene β-actin as an endogenous reference. The fold-increase over the control was calculated using the relative quantification method of 2−ΔΔCt. Mean ± SD, n = 3. (B, C) Flow cytometry analysis of DC phenotypes based on the expression of CD83, CD86 and HLA-DR. Mean ± SD, n = 7. * P<0.05, ** P<0.01 and *** P<0.001 compared with the mDCs. The experiments were conducted in triplicate.