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. 2013 Nov 13;8(11):e78618. doi: 10.1371/journal.pone.0078618

Figure 6. GDF-15 inhibited T cell stimulation and CTL activation induced by DCs.

Figure 6

(A) MLR. DC-mediated T cell stimulation was analyzed in an MLR by incubating DCs obtained under different culture conditions with T lymphocytes at the indicated ratios. (B) At a DC: T cell ratio of 1∶5, the T cell-stimulatory capacity of DCs was significantly reduced by GDF-15. Mean ± SD, n = 4. (C) CTL assay. DCs obtained under different culture conditions were co-cultured with T cells at a ratio of 1∶5 for 3–4 days to generate effector cells. SW480 cells were then co-cultured with the activated T cells at a ratio of 1∶10, 1∶20, 1∶40 or 1∶80 for 36 h. The LDH in the supernatants was detected using a kit. Mean ± SD, n = 8. *** P<0.001 compared with the mDCs. The experiments were conducted in triplicate.