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. 2013 Nov 13;8(11):e78474. doi: 10.1371/journal.pone.0078474

Figure 2. Presence of HERV-Wenv protein on the plasma membrane of PBMC subpopulations from IM patients and controls.

Figure 2

Flow cytometry evaluation of alive PBMC from IM patients (IM) and healthy donors (HD), either with anti-EBNA-1 IgG titers >600 IU/ml or EBV− negative (EBV−). Data were evaluated as percentage of cells with HERV-Wenv-specific staining, as specified in Methods, and are expressed as medians (line), with maximum and minimum values (whiskers); boxes represent interquartile range of the samples. Statistical significance was evaluated by the Mann-Whitney U test for comparison of two groups. When not specified, differences were not significant. All samples (four for each group) were evaluated in the same day. (A) Comparison of HERV-W-specific staining of whole PBMC. (B), (C), (D) HERV-W-specific staining of PBMC subpopulations sorted by specific staining with anti-CD19, anti-CD16 and anti-CD14 antibodies, respectively.